Restriction Enzymes
New England Biolabs, Inc. CviQI – 2000 units
A restriction endonuclease that recognizes the sequence G^TA_C.
New England Biolabs, Inc. Hpy166II – 1000 units
A restriction endonuclease that recognizes the sequence GTN^_NAC.
New England Biolabs, Inc. FatI – 50 units
A restriction endonuclease that recognizes the sequence ^CATG_.
New England Biolabs, Inc. PspOMI – 7500 units
A restriction endonuclease that recognizes the sequence G^GGCC_C.
New England Biolabs, Inc. Nb.BtsI – 1000 units
A restriction endonuclease that recognizes the sequence GCAGTG_NN.
New England Biolabs, Inc. SphI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. SphI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. Esp3I – 1500 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN_....
New England Biolabs, Inc. BclI-HF – 15000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GATCA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. EcoGII Methyltransferase – 200 units
EcoGII Methyltransferase is a non-specific methyltransferase that modifies adenine residues (N6) in any sequence context.
New England Biolabs, Inc. Nb.BssSI – 1000 units
A restriction endonuclease that recognizes the sequence CACGAG.
New England Biolabs, Inc. BsaI-HF®v2 – 1000 units
BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI. Works well for any protocol requiring DNA cutting by BsaI. Recommended enzyme for any purpose requiring digestion at the recognition sequence ...5-GGTCTC(N1)/(N5)-3...
- Engineered for improved performance
- Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer
- Type IIS REs recognize asymmetric DNA sequences; cleave outside their recognition sequence
- 100% activity in rCutSmart Buffer
- Time-Saver qualified for digestion in 5-15 minutes
New England Biolabs, Inc. NruI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence TCG^CGA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. DraIII-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence CAC_NNN^GTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
